ISH (in situ Hybridisation) enables the detection of specific DNA or RNA sequences in histological and cytological specimens, without losing the often very essential morphological details. In case of matching sequences, a hybrid between the probe and target will be formed. Non-perfect matches are washed out by the stringency wash procedure (PanWash). The formed hybrids can easily be visualised by a specific staining procedure, i.e. substrate conversion by enzyme-conjugated antibodies. This conversion, i.e. the combination of NBT/BCIP and Alkaline Phosphatase (AP) conjugated anti-BIO antibodies provided with this kit, will yield a detectable and coloured precipitation. The ISH technique is highly sensitive, specific, fast and easy to perform. Moreover, no radioactivity is involved.
Contents description: |
Contents
|
|
Pepsin digestion reagent Storage: 4-8 °C Assays: 100 |
1 g |
|