Rembrand

BIO-AP Universal RISH & Detection

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This Universal kit for RNA in situ hybridisation and detection is tailored to biotin labelled probes in combination with Alkaline Phosphatase and NBT/BCIP as method of detection.
Base price: 484.00 €
Sales price: 484.00 €
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Description

ISH (in situ Hybridisation) enables the detection of specific DNA or RNA sequences in histological and cytological specimens, without losing the often very essential morphological details. In case of matching sequences, a hybrid between the probe and target will be formed. Non-perfect matches are washed out by the stringency wash procedure (PanWash). The formed hybrids can easily be visualised by a specific staining procedure, i.e. substrate conversion by enzyme-conjugated antibodies. This conversion, i.e. the combination of NBT/BCIP and Alkaline Phosphatase (AP) conjugated anti-BIO antibodies provided with this kit, will yield a detectable and coloured precipitation. The ISH technique is highly sensitive, specific, fast and easy to perform. Moreover, no radioactivity is involved. 

Contents of the kit:
Volume

Pepsin digestion reagent 
Pepsin diluent 
RISH positive control BIO-oligonucleotide probe
RISH negative control BIO-oligonucleotide probe
Anti BIO-AP conjugate
NBT/BCIP substrate
Nuclear fast red counterstain
TBS buffer salt
Coated glass slides
Coverslips

Storage: 4-8 °C                                                              Assays:100

1 g
15 ml
0.8 ml
0.8 ml
15 ml
15 ml
15 ml
2 pcs
50 pcs
100 pcs